Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Campylobacteriosis is a bacterial disease caused by Campylobacter jejuni and Campylobacter coli. These bacteria do not normally cause clinical disease in adult animals except for sporadic cases of abortion in ruminants and very rare cases of hepatitis in ostriches. However, it can be an important source of human food-borne disease through the faecal contamination of meat (especially poultry meat) during processing. The organism can be isolated from faeces, rectal swabs, or caecal contents of mammals (farm animals, cats, and dogs) and birds.
Pathogens Tested
APC-029 :Campylobacter coli
APC-028 :Campylobacter jejuni
Method
Real -Time PCR
Sample Type
Accredited :
Culture.
Alternatives :
Stool, Swab/Secretion (Rectal), Tissue.
Transport Condition
Samples should be transported at 4°C. Stool must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Chlamydia and chlamydophila are two genera belonging to the family Chlamydiaceae. Avian chlamydiosis (caused by Chlamydophila psittaci) was originally termed psittacosis, or parrot fever, as the disease was originally recognised in psittacine birds. Also, Chalmydial disease from domestic poultry and wild birds other than psittacine birds was previously called ornithosis. These diseases are now considered all similar and referred to as avian chlamydiosis.
Chlamydiae are known to infect most species of domestic poultry, pet birds and wild birds causing varying degrees of infection. Chlamydial infections have been identified in over 150 species of wild birds. Persistently infected carrier birds are known to be a source of chlamydiosis in the pet bird industry. In poultry, the disease varies from one producing high morbidity and mortality to one that is asymptomatic. Typical clinical signs with a strain of high virulence include pneumoenteritis with respiratory signs, mucopurulent ocular or nasal discharge, conjunctivitis, diarrhoea, polyuria and dullness. Strains of low virulence produce clinical signs that are similar but less severe and less extensive. Asymptomatic infections can occur with strains of both low and high virulence.
Infected birds shed chlamydiae in both the respiratory excretions and in faeces. A susceptible bird can become infected through inhalation of airborne contaminated material or through ingestion of contaminated feeds.
Samples should be transported at 4°C. Stool must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
The C. perfringens species is a very heterogeneous group of organisms with respect to their metabolic byproducts, toxins and pathogenic potential. C. perfringens is classified into five toxigenic types (A through E), based on their ability to produce any of the four major lethal toxins alpha, beta, epsilon and iota. However, there is a considerable lack of knowledge regarding the distribution of C. perfringens types, their pathogenesis, diagnosis and the incidence of diseases caused by this organism.
Type A is the most common of all the C. perfringens types and the most variable in toxigenic properties. Its role in the pathogenesis of diseases is not fully understood. Type A can be subdivided into two varieties based on its toxigenic behaviour; the "classical" variety, characterized mainly by alphatoxin production and is associated with gas gangrene, traumatic infections, avian necrotic enteritis and the normal intestinal tract, and the enterotoxigenic variety, characterized by enterotoxin production and capable of causing human enteritis.
Type B produces both beta- and epsilon-toxins, but beta-toxin is usually the principal component.
The principal lethal toxin in all varieties of C. perfringens type C is beta-toxin. The enterotoxigenic C. perfringens appears to originate from animals since most of the sources incriminated in food poisoning outbreaks have been meats, particularly beef and poultry.
Type D is the best known pathogenic C. perfringens type and widely regarded as the causative organism of fatal enterotoxemia of sheep or "overeating disease". It produces epsilon-toxin which is almost exclusively responsible for the host pathology and subsequent death.
This assay can detect alpha, beta-2, epsilon, iota and enterotoxin.
Method
Multi Real-Time PCR.
Sample Type
Stool, Swab / Secretion (Rectal), Culture.
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Corynebacterium pseudotuberculosis is a gram-positive, facultative intracellular pathogen and the etiologic agent
of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep. It also causes ulcerative lymphangitis, external subcutaneous abscesses, and internal infection in horses. Infection has also been reported in cattle, buffalo and camelids. In camels, C. pseudotuberculosis affects almost 10% of the population in a herd.
Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity.
Method
PCR & Gel Electrophoresis
Sample Type
Culture, EDTA Blood, Tissue.
Transport Condition
Sample should be transported at 4°C. Milk must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Coxiella burnetii is a gram negative cocco bacillus that causes Q-fever disease in animals. It belongs to a group of organisms known as Rickettsia. The infection has been found in various wild and domestic animals and birds and in some arthropods, such as ticks. The species most commonly infected are cattle, sheep and goats. Infections with Coxiella burnetii include placentitis (inflammation of the placenta) and subsequent abortion in cattle, sheep and goats.
Outside the animal the bacteria assumes a small, dense, long lasting spore-like form which is able to resist heat and drying. It can then contaminate dust and spread by wind for long distances. It is so highly infectious that a single inhaled organism can cause clinical illness in an animal or person. Outbreaks typically occur following a birth or abortion where the environment becomes contaminated with birth fluids. Q-fever can also be spread by ticks which pass the bacteria from an infected to a susceptible animal, and whose feces contain the bacteria thus also contaminating the environment. The organism may be present in the reproductive fluids or raw milk from infected animals. Animal vaccination has been used in areas where the infections are common. More generally, sanitary measures to remove afterbirth and birth fluids, and to clean and disinfect areas where animals have given birth can prevent the disease from spreading.
Q-fever is listed in the OIE Terrestrial Animal Health Code and Member Countries and Territories are obligated to report occurrences of the disease to the OIE. This assay is used for the detection of both C. burnetii and C. symbiont, however, it cannot differentiate between the two subtypes.
Method
Real -Time PCR
Sample Type
Accredited :
Culture, EDTA Blood, Milk, Tissue.
Alternatives :
Swab / Secretion (Genital).
Transport Condition
Samples should be transported at 4°C. Milk must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Campylobacteriosis is a bacterial disease caused by Campylobacter jejuni and Campylobacter coli. These bacteria do not normally cause clinical disease in adult animals except for sporadic cases of abortion in ruminants and very rare cases of hepatitis in ostriches. However, it can be an important source of human food-borne disease through the faecal contamination of meat (especially poultry meat) during processing. The organism can be isolated from faeces, rectal swabs, or caecal contents of mammals (farm animals, cats, and dogs) and birds.
Pathogens Tested
APC-029 :Campylobacter coli
APC-028 :Campylobacter jejuni
Method
Real -Time PCR
Sample Type
Accredited :
Culture.
Alternatives :
Stool, Swab/Secretion (Rectal), Tissue.
Transport Condition
Samples should be transported at 4°C. Stool must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Chlamydia and chlamydophila are two genera belonging to the family Chlamydiaceae. Avian chlamydiosis (caused by Chlamydophila psittaci) was originally termed psittacosis, or parrot fever, as the disease was originally recognised in psittacine birds. Also, Chalmydial disease from domestic poultry and wild birds other than psittacine birds was previously called ornithosis. These diseases are now considered all similar and referred to as avian chlamydiosis.
Chlamydiae are known to infect most species of domestic poultry, pet birds and wild birds causing varying degrees of infection. Chlamydial infections have been identified in over 150 species of wild birds. Persistently infected carrier birds are known to be a source of chlamydiosis in the pet bird industry. In poultry, the disease varies from one producing high morbidity and mortality to one that is asymptomatic. Typical clinical signs with a strain of high virulence include pneumoenteritis with respiratory signs, mucopurulent ocular or nasal discharge, conjunctivitis, diarrhoea, polyuria and dullness. Strains of low virulence produce clinical signs that are similar but less severe and less extensive. Asymptomatic infections can occur with strains of both low and high virulence.
Infected birds shed chlamydiae in both the respiratory excretions and in faeces. A susceptible bird can become infected through inhalation of airborne contaminated material or through ingestion of contaminated feeds.
Samples should be transported at 4°C. Stool must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
The C. perfringens species is a very heterogeneous group of organisms with respect to their metabolic byproducts, toxins and pathogenic potential. C. perfringens is classified into five toxigenic types (A through E), based on their ability to produce any of the four major lethal toxins alpha, beta, epsilon and iota. However, there is a considerable lack of knowledge regarding the distribution of C. perfringens types, their pathogenesis, diagnosis and the incidence of diseases caused by this organism.
Type A is the most common of all the C. perfringens types and the most variable in toxigenic properties. Its role in the pathogenesis of diseases is not fully understood. Type A can be subdivided into two varieties based on its toxigenic behaviour; the "classical" variety, characterized mainly by alphatoxin production and is associated with gas gangrene, traumatic infections, avian necrotic enteritis and the normal intestinal tract, and the enterotoxigenic variety, characterized by enterotoxin production and capable of causing human enteritis.
Type B produces both beta- and epsilon-toxins, but beta-toxin is usually the principal component.
The principal lethal toxin in all varieties of C. perfringens type C is beta-toxin. The enterotoxigenic C. perfringens appears to originate from animals since most of the sources incriminated in food poisoning outbreaks have been meats, particularly beef and poultry.
Type D is the best known pathogenic C. perfringens type and widely regarded as the causative organism of fatal enterotoxemia of sheep or "overeating disease". It produces epsilon-toxin which is almost exclusively responsible for the host pathology and subsequent death.
This assay can detect alpha, beta-2, epsilon, iota and enterotoxin.
Method
Multi Real-Time PCR.
Sample Type
Stool, Swab / Secretion (Rectal), Culture.
Transport Condition
Samples should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Corynebacterium pseudotuberculosis is a gram-positive, facultative intracellular pathogen and the etiologic agent
of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep. It also causes ulcerative lymphangitis, external subcutaneous abscesses, and internal infection in horses. Infection has also been reported in cattle, buffalo and camelids. In camels, C. pseudotuberculosis affects almost 10% of the population in a herd.
Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity.
Method
PCR & Gel Electrophoresis
Sample Type
Culture, EDTA Blood, Tissue.
Transport Condition
Sample should be transported at 4°C. Milk must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Coxiella burnetii is a gram negative cocco bacillus that causes Q-fever disease in animals. It belongs to a group of organisms known as Rickettsia. The infection has been found in various wild and domestic animals and birds and in some arthropods, such as ticks. The species most commonly infected are cattle, sheep and goats. Infections with Coxiella burnetii include placentitis (inflammation of the placenta) and subsequent abortion in cattle, sheep and goats.
Outside the animal the bacteria assumes a small, dense, long lasting spore-like form which is able to resist heat and drying. It can then contaminate dust and spread by wind for long distances. It is so highly infectious that a single inhaled organism can cause clinical illness in an animal or person. Outbreaks typically occur following a birth or abortion where the environment becomes contaminated with birth fluids. Q-fever can also be spread by ticks which pass the bacteria from an infected to a susceptible animal, and whose feces contain the bacteria thus also contaminating the environment. The organism may be present in the reproductive fluids or raw milk from infected animals. Animal vaccination has been used in areas where the infections are common. More generally, sanitary measures to remove afterbirth and birth fluids, and to clean and disinfect areas where animals have given birth can prevent the disease from spreading.
Q-fever is listed in the OIE Terrestrial Animal Health Code and Member Countries and Territories are obligated to report occurrences of the disease to the OIE. This assay is used for the detection of both C. burnetii and C. symbiont, however, it cannot differentiate between the two subtypes.
Method
Real -Time PCR
Sample Type
Accredited :
Culture, EDTA Blood, Milk, Tissue.
Alternatives :
Swab / Secretion (Genital).
Transport Condition
Samples should be transported at 4°C. Milk must be frozen after collection.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
